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- Memoirs of the Department of Agriculture in India
Memoirs of the Department of Agriculture in India
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Excerpt from Memoirs of the Department of Agriculture in India: Studies on the Root Nodule Organism of the Leguminous Plants
Different views are again expressed on the mechanism of nitrogen fixation or as to how the organism is enabled to fix atmospheric nitrogen and what is the first product of synthesis.
Besides these fundamental questions of practical importance, there are a number of other minor issues on which opinion is not unanimous. This state of affairs is partly due to contradictory results obtained by different investi gators working under diverse conditions and each one of them making general deductions based on work which was carried out under a particular set of conditions. Although in a complicated problem involving several factors this result is not unexpected, it shows the necessity of a still greater amount of work under conditions which can be controlled at will, without which it would not be possible to explain or reconcile the extreme views held by different workers.
The present writer had to demonstrate some work in connection with this subject to the students in this laboratory, and the opportunity was taken to investigate the question of species by cross-inoculation of the organism from root nodules of one plant on another, and the nitrogen-fixing power of the organism independent of the plant under laboratory conditions. In the course of this work some interesting results were obtained and so further work on the subject was done during the last few years. It is not claimed that the work is in any way complete. The chief object in putting together the results obtained so far is, however, to elicit criticism of methods employed, and in the light of that criticism to know the exact position reached with regard to the subject.
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