B Cell Receptor Signaling

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This volume looks at the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution, interactions between B cells and viruses, mechanical force during BCR activation, B cell signaling using flow cytometry, confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy, and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently workingin the B cell field.

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