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- Effect of organic solutes on heat denaturation of proteins
Effect of organic solutes on heat denaturation of proteins
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INTRODUCTION
Since the classic experiments of Anfinsen on ribonuclease A, it has been widely accepted that the three-dimensional structure of a protein is determined by its amino acid sequence (Anfinsen et al... 1961). The conformational stability (AGD) of a protein can be defined as the free energy change for the equilibrium reaction,
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