Lipid Second Messengers

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Lipid Second Messengers provides detailed methodology for analysis of various lipid signaling pathways. Authoritative contributors explain the factors that regulate lipid second messenger production by agonist-activated enzymes and examine their products. Topics discussed include procedures used to measure lipid-derived mediators such as lysophospholipids, arachidonic acid, eicosanoids, anandamide, and ceramides, and the enzymes responsible for generating these messengers, such as phospholipases, prostaglandin endoperoxide synthases, and sphingomyelinase. TOC:Phospholipase D.- Analyzing the Sphingomyelin Cycle: Protocols for Measuring Sphingomyelinase, Sphingomyelin, and Ceramide.- Expression of the Human Prostaglandin Endoperoxide H Synthases: Measurement of Cyclooxygenase Activity and Inhibition by Non-Steroidal Antiinflammatory Drugs.- Phospholipid Growth Factors: Identification and Mechanisms of Action.- Leukotriene and Lipoxin Biosynthesis.- Purification and High-Resolution Analysis of Anandamide and Other Fatty Acylethanolamides.

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