Methods for Investigation of Amino Acid and Protein Metabolism

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No other current book demonstrates detailed research methods in the field of amino acid and protein nutrition and metabolism. Containing all the new as well as classical methodologies used in the investigation of amino acid and protein metabolism in human and animal models, this book is needed because of the dramatic increase in research in this field. Methods for Investigation of Amino Acid and Protein Metabolism explores areas such as amino acid transfer across tissue membranes, past and new applications using stable isotopes, protein synthesis in organs and tissues, and more. Because of the importance of research methods in the field of amino acid and protein nutrition and metabolism, this book facilitates the reader's integration of the concepts involved in these investigative research methods and their corollaries. In addition to helping any nutrition investigator design and conduct appropriate research protocols in this area of nutrition, this book assists students who are planning to investigate amino acid and protein metabolism in humans or laboratory animals. TOC:Investigation of Amino Acid Transfer Across Tissue Membranes.- Whole-Body Protein Turnover in Humans: Past and New Applications Using Stable Isotopes.- Protein Synthesis in Organs and Tissues: Quantitative Methods in Laboratory Animals.- The Measurement of the Rate of Synthesis of Liver-Derived Plasma Proteins.- Evaluation of the Adaptation to Protein Restriction in Humans.- Determination of Amino Acid Isotopic Enrichment: Methods, Difficulties, and Calculations.- The Use of GC-C-IRMS for the Analysis of Stable Isotope Enrichment in Nitrogenous Compounds.- Use of Isotope Dilution Methods to Investigate Glutamine Metabolism In Vivo in Humans.- The Application of Muscle Biopsy in the Study of Amino Acid and Protein Metabolism.- Protein and Amino Acid Metabolism in the Elderly.- Evaluation of Amino Acid and Protein Metabolism in the Fetus.- Analysis of Proteins in Human Milk.- Studying Amino Acid and Protein Metabolism in Burn Patients.- Methods for Investigation of Protein and Amino Acid Metabolism in Diabetes Mellitus.