Prostate Cancer Methods and Protocols

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With the prostate as the second leading site of cancer among males in Western society, the need to know why certain prostate cancers remain localized while others become aggressive and metastasize has become critical for the care of patients. In Prostate Cancer Methods and Protocols, leading investigators present basic and advanced molecular methods that reveal those markers essential for more accurate diagnoses of specific diseases, and in developing new treatment strategies. The techniques range from in vitro methods to in vivo models of prostate cancer. New methods for the accurate diagnosis of prostate cancer are also offered, including histological assessment, studies of cells in semen, and methylation analysis of the GSTpi, a potential prostate cancer-specific marker. Additional approaches, including proteome and microarray analyses, address such issues as assessing changes in the androgen receptor and studying the enzymes secreted by prostate cancer cells. New strategies for the treatment of refractory disease cover the role of flavonoids, targeted alpha therapy, methods for implementing immune therapy, and prostate-specific gene therapy. Each method is described in step-by-step detail to ensure successful results. Comprehensive and cutting-edge, Prostate Cancer Methods and Protocols provides access to the many powerful tools needed by scientists engaged in prostate cancer research today, as well as up-to-date reviews on several key aspects of the disease.