Manual of Immunological Methods

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The Manual of Immunological Methods provides detailed immunological methods that can be utilized by researchers or practitioners who want to enhance the successful application of this science. It emphasizes the need for continuously improving the quality of experiments performed and maintaining consistency in the results obtained. The book emphasizes the need for continuously improving the quality of experiments performed It covers specific methodologies including sample collection, preparation of cells, assay conditions, and data evaluation. Discussion of species differences and comparisons of a wide variety of species is an important feature. TOC:Identification, Anaesthesia, and Euthanasia. Collection of Peripheral Blood Samples. Removal of Organs. Preparation of Cell Suspensions. Assessment of Cell Viability. Cell Cryopreservation. Oxidative Burst Assay Using Flow Cytometry. Cell Cytotoxicity. Lymphoblastic Transformation. Determination of Antibody-Producing Cells to a Specific Antigen. Mixed Lymphocyte Reaction (MLR). Intracellular Levels of Calcium Assay Using Flow Cytometry. Phenotyping of Blood Mononuclear Cells. Evaluation of Intracellular Level of Thiols. Apoptosis.